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Electron microscopic imaging and NanoSIMS investigation on physiological responses of Aspergillus niger under Pb(II) and Cd(II) stress
Pan, Shang1,2,3; Li, Zhaoyan2; Wang, Jiayi2; Li, Xuefei2; Meng, Lingzi2; Chen, Yunhui2; Su, Mu2; Li, Zhen(李真)2,3,4
2023-01-12
Source PublicationFRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
ISSN2296-4185
Volume10Pages:10
Abstract

In the bioremediation process, coexistence of lead (Pb) and cadmium causes complex toxicity, resulting in the difficulty of bioremediation. This study investigated the physiological responses and bioaccumulation mechanisms of the typical filamentous fungus Aspergillus niger under the coexistence of Pb and Cd. Four treatments were set up, i.e., control, sole Pb, sole Cd, and coexistence of Pb and Cd. The morphology of A. niger were observed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM), respectively. Then, nano-scale secondary ion mass spectrometry (NanoSIMS) was applied to accurately investigate the distribution of heavy metals in the fungal cells under the coexistence of Pb and Cd. Finally, the metallogenic process and mineral types were simulated by Geochemist's Workbench (GWB). The electron microscopic and NanoSIMS imaging showed that Pb and Cd were accumulated in both the extracellular and intracellular regions of the A. niger cells. In particular, the accumulated Pb content was ten times higher than that of Cd. However, Cd showed stronger toxicity than Pb to A. niger. Compared with the control treatment, Cd stress resulted in a two-fold increase of cell diameter and more extracellular substances, whereas the cell diameter increased nearly four times in the coexistence treatment. Moreover, the bioaccumulation of Pb was more intense than that of Cd during competitive sorption. The GWB simulation confirmed that Pb2+ can form multiple minerals (e.g., PbC2O4, PbHPO4, and Pb-3(PO4)(2), etc.), which significantly weakened its toxicity on the cell surface. This study elucidated the morphological characteristics of A. niger and competitive bioaccumulation under the coexistence of Pb and Cd, which would facilitate the application of microorganisms to the bioremediation of coexisted metals.

Keywordlead cadmium Aspergillus niger electron microscopy NanoSIMS GWB simulation
DOI10.3389/fbioe.2022.1096384
Indexed BySCI
Language英语
WOS KeywordEXTRACELLULAR POLYMERIC SUBSTANCES ; HEAVY-METALS ; LEAD ; SOIL ; BIOSORPTION ; PB ; REMOVAL ; CADMIUM ; IMMOBILIZATION ; MINERALS
Funding ProjectFundamental Research Funds for the Central Universities[KYCYXT2022004] ; State Key Laboratory of Palaeobiology and Stratigraphy (Nanjing Institute of Geology and Palaeontology, CAS)[223116]
WOS Research AreaBiotechnology & Applied Microbiology ; Science & Technology - Other Topics
WOS SubjectBiotechnology & Applied Microbiology ; Multidisciplinary Sciences
WOS IDWOS:000920250100001
Funding OrganizationFundamental Research Funds for the Central Universities ; State Key Laboratory of Palaeobiology and Stratigraphy (Nanjing Institute of Geology and Palaeontology, CAS)
PublisherFRONTIERS MEDIA SA
Document Type期刊论文
Identifierhttp://ir.nigpas.ac.cn/handle/332004/41706
Collection中国科学院南京地质古生物研究所
Corresponding AuthorPan, Shang; Li, Zhen(李真)
Affiliation1.Nanjing Agr Univ, Coll Agro Grassland Sci, Nanjing, Peoples R China
2.Nanjing Agr Univ, Coll Resources & Environm Sci, Nanjing, Peoples R China
3.Nanjing Agr Univ, Jiangsu Key Lab Organ Waste Utilizat, Nanjing, Peoples R China
4.Nanjing Inst Geol & Palaeontol, State Key Lab Palaeobiol & Stratig, Nanjing, Peoples R China
Corresponding Author AffilicationNanjing Institute of Geology and Palaeonotology,CAS
Recommended Citation
GB/T 7714
Pan, Shang,Li, Zhaoyan,Wang, Jiayi,et al. Electron microscopic imaging and NanoSIMS investigation on physiological responses of Aspergillus niger under Pb(II) and Cd(II) stress[J]. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY,2023,10:10.
APA Pan, Shang.,Li, Zhaoyan.,Wang, Jiayi.,Li, Xuefei.,Meng, Lingzi.,...&Li, Zhen.(2023).Electron microscopic imaging and NanoSIMS investigation on physiological responses of Aspergillus niger under Pb(II) and Cd(II) stress.FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY,10,10.
MLA Pan, Shang,et al."Electron microscopic imaging and NanoSIMS investigation on physiological responses of Aspergillus niger under Pb(II) and Cd(II) stress".FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY 10(2023):10.
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