KMS Nanjing Institute of Geology and Palaeonotology,CAS
Programmed cell death during pigment gland formation in Gossypium hirsutum leaves | |
Liu, W. -Z.1; Zhou, Y. -F.; Wang, X. (王鑫)2; Jiao, Z. -J. | |
2010-11-01 | |
发表期刊 | PLANT BIOLOGY |
ISSN | 1435-8603 |
卷号 | 12期号:6页码:895-902 |
摘要 | Ultrastructural studies have shown that the formation of pigment glands in Gossypium hirsutum L. leaves is a lysigenous process, originating from a cluster of cells in the ground meristem. Various techniques were used here to investigate whether programmed cell death (PCD) plays a critical role in this developmental process. Nuclei of internal cells in the pigment gland-forming tissue were TUNEL-positive and DAPI-negative, suggesting that DNA cleavage is an early event and complete DNA degradation is a late event. Smeared bands and a lack of laddering after gel electrophoresis indicate that DNA cleavage is random. Ultrastructurally, secretory cells in the pigment glands become distorted, nuclei are densely stained, and chromosomes become condensed until completely degraded at late stages. Vacuoles with electron-dense bodies and membrane-bound autophagosomes are seen in both secretory and sheath cells, suggesting that autophagy plays a key role in PCD during cytoplasm degradation. Buckling of cell walls, seen at early stages, later leads to a complete breakdown of the walls. Together, these results suggest that PCD plays a critical role in the lysigenous development of pigment glands in G. hirsutum leaves. |
关键词 | Autophagosome Dapi Assay Gossypium Hirsutum l Pigment Gland Programmed Cell Death Tunel Assay |
DOI | 10.1111/j.1438-8677.2009.00291.x |
语种 | 英语 |
关键词[WOS] | Floral Nectary ; Plants ; Cotton ; L. ; Purpurea ; Ontogeny ; Defense ; Maize |
资助项目 | National Natural Science Foundation of China[30970170] ; Shannxi Provincial Department of Education, Scientific Research Projects for Key Disciplines of the Key Laboratory of China[09JS087] |
WOS研究方向 | Plant Sciences |
WOS类目 | Plant Sciences |
WOS记录号 | WOS:000282817700007 |
项目资助者 | National Natural Science Foundation of China ; Shannxi Provincial Department of Education, Scientific Research Projects for Key Disciplines of the Key Laboratory of China |
出版者 | WILEY-BLACKWELL |
文献类型 | 期刊论文 |
条目标识符 | http://ir.nigpas.ac.cn/handle/332004/395 |
专题 | 中国科学院南京地质古生物研究所 其他 |
通讯作者 | Liu, W. -Z. |
作者单位 | 1.Northwest Univ, Sch Life Sci, Key Lab Resource Biol & Biotechnol Western China, Minist Educ, Xian 710069, Peoples R China 2.Nanjing Inst Geol & Palaeontol, State Key Lab Palaeobiol & Stratig, Nanjing, Peoples R China |
推荐引用方式 GB/T 7714 | Liu, W. -Z.,Zhou, Y. -F.,Wang, X. ,et al. Programmed cell death during pigment gland formation in Gossypium hirsutum leaves[J]. PLANT BIOLOGY,2010,12(6):895-902. |
APA | Liu, W. -Z.,Zhou, Y. -F.,Wang, X. ,&Jiao, Z. -J..(2010).Programmed cell death during pigment gland formation in Gossypium hirsutum leaves.PLANT BIOLOGY,12(6),895-902. |
MLA | Liu, W. -Z.,et al."Programmed cell death during pigment gland formation in Gossypium hirsutum leaves".PLANT BIOLOGY 12.6(2010):895-902. |
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