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Comparative transcriptomic analysis of fireflies (Coleoptera: Lampyridae) to explore the molecular adaptations to fresh water
Zhang, Qi-Lin1; Li, Hong-Wei1; Dong, Zhi-Xiang1; Yang, Xiao-Jie1; Lin, Lian-Bing1; Chen, Jun-Yuan (陈均远)3; Yuan, Ming-Long2
2020-06-26
Source PublicationMOLECULAR ECOLOGY
ISSN0962-1083
Pages16
Abstract

Aquatic insects are well adapted to freshwater environments, but the molecular basis of these adaptations remains largely unknown. Most firefly species (Coleoptera: Lampyridae) are terrestrial, but the larvae of several species are aquatic. Here, larval and adult transcriptomes fromAquatica leii(freshwater) andLychnuris praetexta(terrestrial) were generated to test whether the genes associated with metabolic efficiency and morphology have undergone adaptive evolution to fresh water. The aquatic fireflies had a significantly lower ratio of nonsynonymous to synonymous substitutions than the terrestrial insects, indicating a genomewide evolutionary constraint in the aquatic fireflies. We identified 341 fast-evolving genes and 116 positively selected genes in the aquatic fireflies. Of these, 76 genes exhibiting both fast evolution and positive selection were primarily involved in ATP production, energy metabolism and the hypoxia response. We identified 7,271 differentially expressed genes (DEGs) inA. leii(adults versus larvae) and 8,309 DEGs inL. praetexta(adults versus larvae). DEGs specific to the aquatic firefly (n = 1,445) were screened via interspecific comparisons (A. leiiversusL. praetexta) and were significantly enriched for genes involved in metabolic efficiency (e.g., ATP production, hypoxia, and immune responses) and certain aspects of morphology (e.g., cuticle chitin, tracheal and compound eye morphology). These results indicate that sequence and expression-level changes in genes associated with both metabolic efficiency and morphological attributes related to the freshwater lifestyle contributed to freshwater adaptation in fireflies. This study provides new insights into the molecular mechanisms of aquatic adaptation in insects.

Keywordaquatic firefly comparative transcriptomics freshwater environment metabolic efficiency molecular adaptation morphology
DOI10.1111/mec.15504
Indexed BySCI
Language英语
WOS KeywordPHYLOGENETIC ANALYSIS ; ADAPTIVE EVOLUTION ; ANALYSIS REVEALS ; HYPOXIA ; ANNOTATION ; GYNAEPHORA ; EXPRESSION ; GENES ; RNA ; REGULATOR
Funding ProjectNatural Science Foundation of China[31960286]
WOS Research AreaBiochemistry & Molecular Biology ; Environmental Sciences & Ecology ; Evolutionary Biology
WOS SubjectBiochemistry & Molecular Biology ; Ecology ; Evolutionary Biology
WOS IDWOS:000543234300001
Funding OrganizationNatural Science Foundation of China
PublisherWILEY
Citation statistics
Document Type期刊论文
Identifierhttp://ir.nigpas.ac.cn/handle/332004/32056
Collection中国科学院南京地质古生物研究所
Corresponding AuthorZhang, Qi-Lin; Chen, Jun-Yuan (陈均远); Yuan, Ming-Long
Affiliation1.Kunming Univ Sci & Technol, Fac Life Sci & Technol, Kunming, Yunnan, Peoples R China
2.Lanzhou Univ, Coll Pastoral Agr Sci & Technol, State Key Lab Grassland Agroecosyst, Lanzhou, Peoples R China
3.Chinese Acad Sci, Nanjing Inst Geol & Paleontol, LPS, Nanjing, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Qi-Lin,Li, Hong-Wei,Dong, Zhi-Xiang,et al. Comparative transcriptomic analysis of fireflies (Coleoptera: Lampyridae) to explore the molecular adaptations to fresh water[J]. MOLECULAR ECOLOGY,2020:16.
APA Zhang, Qi-Lin.,Li, Hong-Wei.,Dong, Zhi-Xiang.,Yang, Xiao-Jie.,Lin, Lian-Bing.,...&Yuan, Ming-Long.(2020).Comparative transcriptomic analysis of fireflies (Coleoptera: Lampyridae) to explore the molecular adaptations to fresh water.MOLECULAR ECOLOGY,16.
MLA Zhang, Qi-Lin,et al."Comparative transcriptomic analysis of fireflies (Coleoptera: Lampyridae) to explore the molecular adaptations to fresh water".MOLECULAR ECOLOGY (2020):16.
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