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Genome-wide gene expression analysis in the amphioxus, Branchiostoma belcheri after poly (I: C) challenge using strand-specific RNA-seq
Zhang, Qi-Lin1,2; Xie, Zheng-Qing1,2; Liang, Ming-Zhong3; Luo, Bang4; Wang, Xiu-Qiang1; Chen, Jun-Yuan(陈均远)1
2017-12-12
Source PublicationONCOTARGET
ISSN1949-2553
Volume8Issue:65Pages:108392-108405
Abstract

The gene expression associated with immune response to bacteria/bacterial mimic has been extensively analyzed in amphioxus, but remains largely unknown about how gene are involved in the immune response to viral invasion at expression level. Here, we analyze the rRNA-depleted transcriptomes of Branchiostoma belcheri using strand-specific RNA-seq in response to the viral mimic, poly (I:C) (pIC). A total of 5,317 differentially expressed genes were detected at treatment group by comparing with control. The gene with the most significant expression changes (top 15) after pIC challenge and 7 immune-related categories involving 58 differently expressed genes were scrutinized. By functional enrichment analysis of differently expressed genes, gene ontology terms involving response to stress and stimulus, apoptosis, catabolic and metabolic processes and enzyme activity were overrepresented, and several pathways related to immune signaling, immune response, cancer, apoptosis, viral disease, metabolism were activated after pIC injection. A positive correlation between the qRT-PCR and strand-specific RNA-seq data confirmed the accuracy of the RNA-seq results. Additionally, the expression of genes encoding NLRC5, CASP1, CASP6, CYP450, CAT, and MDA5 were induced in B. belcheri under pIC challenge. Our experiments provide insight into the immune response of amphioxus to pIC and valuable gene expression information for studying the evolution of antiviral immunity in vertebrates.

KeywordBranchiostoma Belcheri Transcriptome Analysis Strand-specific Rna-seq Poly (i:c) Antiviral Immunity Immunology
DOI10.18632/oncotarget.21553
Language英语
WOS KeywordNf-kappa-b ; Antiviral Response ; Noncoding Rnas ; Evolution ; Identification ; Japonicum ; Receptor ; Protein ; Cells ; Fish
Funding ProjectMinistry of Science and Technology of China[2013CB835300] ; Natural Science Foundation of China[41272008]
WOS Research AreaOncology ; Cell Biology
WOS SubjectOncology ; Cell Biology
WOS IDWOS:000419565400010
Funding OrganizationMinistry of Science and Technology of China ; Natural Science Foundation of China
PublisherIMPACT JOURNALS LLC
Citation statistics
Document Type期刊论文
Identifierhttp://ir.nigpas.ac.cn/handle/332004/18906
Collection中国科学院南京地质古生物研究所
其他
Corresponding AuthorZhang, Qi-Lin; Chen, Jun-Yuan(陈均远)
Affiliation1.Chinese Acad Sci, Nanjing Inst Geol & Paleontol, LPS, Nanjing, Jiangsu, Peoples R China
2.Nanjing Univ, Sch Life Sci, State Key Lab Pharmaceut Biotechnol, Nanjing, Jiangsu, Peoples R China
3.Qinzhou Univ, Dept Marine Sci, Qinzhou, Peoples R China
4.Guangxi Acad Fishery Sci, Nanning, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Qi-Lin,Xie, Zheng-Qing,Liang, Ming-Zhong,et al. Genome-wide gene expression analysis in the amphioxus, Branchiostoma belcheri after poly (I: C) challenge using strand-specific RNA-seq[J]. ONCOTARGET,2017,8(65):108392-108405.
APA Zhang, Qi-Lin,Xie, Zheng-Qing,Liang, Ming-Zhong,Luo, Bang,Wang, Xiu-Qiang,&Chen, Jun-Yuan.(2017).Genome-wide gene expression analysis in the amphioxus, Branchiostoma belcheri after poly (I: C) challenge using strand-specific RNA-seq.ONCOTARGET,8(65),108392-108405.
MLA Zhang, Qi-Lin,et al."Genome-wide gene expression analysis in the amphioxus, Branchiostoma belcheri after poly (I: C) challenge using strand-specific RNA-seq".ONCOTARGET 8.65(2017):108392-108405.
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