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Forensically Relevant Blow Flies in Lebanon Survey and Identification Using Molecular Markers (Diptera: Calliphoridae)
Shayya, Salman1,2,3,6; Debruyne, Regis4; Nel, Andre1; Azar, Dany5,6
2018-09-01
发表期刊JOURNAL OF MEDICAL ENTOMOLOGY
ISSN0022-2585
卷号55期号:5页码:1113-1123
摘要

Calliphoridae are among the first insects associated to decomposing animal remains. We have collected 1,841 specimens of three calliphorid genera: Calliphora, Lucilia, and Chrysomya, from different Lebanese localities as a first step in implementing a database of insects of forensic relevance for the country. Blow-flies are crucial for the estimation of the postmortem interval. DNA-based identification is a rapid and accurate method, often used for morphologically similar species, especially for immatures or incomplete specimens. In this study, we test the suitability of three genetic markers to identify adults and immature stages of calliphorids, viz., mitochondrial cytochrome c oxidase subunit I (COI) barcode, a region including partial sequences of mitochondrial Cyt-b-tRNA(ser)-ND1, and second internal transcribed spacer (ITS2) region of nuclear ribosomal DNA. Forty Lebanese specimens of various developmental stages (egg, larva, wandering third instar, pupa, newly emerged adult, and mature adult) were identified among the three calliphorid genera: Calliphora, Lucilia, and Chrysomya, and compared with published sequences to confirm their specific assignation. Phylogenetic analyses showed the robustness of ITS2 and COI to identify calliphorids at species level. Nevertheless, ITS2 failed to discriminate Lucilia caesar (Linnaeus) (Diptera, Calliphoridae) from Lucilia illustris (Meigen) (Diptera, Calliphoridae), and COI had a similar issue with Lucilia sericata (Meigen) (Diptera, Calliphoridae) and Lucilia cuprina (Wiedemann) (Diptera, Calliphoridae). Thus, these two markers are complementary. This work contributes new nucleotide sequences for Lebanon. It is a first step in implementing a molecular database of forensic relevant insects for the country.

关键词Forensic Entomology Cytochrome Oxidase i Second Internal Transcribed Spacer Calliphoridae Diagnostic
DOI10.1093/jme/tjy068
语种英语
关键词[WOS]Dna-based Identification ; Cytochrome-oxidase-i ; Mediterranean Blowflies ; Rabbit Carcasses ; 1st Instars ; Species Identification ; Lucilia-sericata ; Morphology ; Succession ; Mitochondrial
资助项目CNRS (National Council of Scientific Research-Lebanon) ; W alid Joumblatt Foundation for University studies
WOS研究方向Entomology ; Veterinary Sciences
WOS类目Entomology ; Veterinary Sciences
WOS记录号WOS:000456130900007
项目资助者CNRS (National Council of Scientific Research-Lebanon) ; W alid Joumblatt Foundation for University studies
出版者OXFORD UNIV PRESS INC
文献类型期刊论文
条目标识符http://ir.nigpas.ac.cn/handle/332004/16070
专题中国科学院南京地质古生物研究所
通讯作者Shayya, Salman
作者单位1.Sorbonne Univ, MNHN, UMR 7205, ISYEB,CNRS,UPMC,EPHE, 57 Rue Cuvier,CP 50, F-75005 Paris, France
2.Rafic Hariri Univ Campus, Doctoral Sch Sci & Technol, Hadath, Lebanon
3.Lebanese Atom Energy Commiss, Natl Council Sci Res Lebanon CNRS L, Beirut, Lebanon
4.Sorbonne Univ, MNHN, CNRS, Serv Systemat Mol,UMS 2700, 57 Rue Cuvier,CP 26, F-75005 Paris, France
5.Chinese Acad Sci, State Key Lab Palaeobiol & Stratig, Nanjing Inst Geol & Palaeontol, Nanjing 210008, Jiangsu, Peoples R China
6.Lebanese Univ, Fac Sci 2, Dept Nat Sci, POB 26110217, Fanar Matn, Lebanon
推荐引用方式
GB/T 7714
Shayya, Salman,Debruyne, Regis,Nel, Andre,et al. Forensically Relevant Blow Flies in Lebanon Survey and Identification Using Molecular Markers (Diptera: Calliphoridae)[J]. JOURNAL OF MEDICAL ENTOMOLOGY,2018,55(5):1113-1123.
APA Shayya, Salman,Debruyne, Regis,Nel, Andre,&Azar, Dany.(2018).Forensically Relevant Blow Flies in Lebanon Survey and Identification Using Molecular Markers (Diptera: Calliphoridae).JOURNAL OF MEDICAL ENTOMOLOGY,55(5),1113-1123.
MLA Shayya, Salman,et al."Forensically Relevant Blow Flies in Lebanon Survey and Identification Using Molecular Markers (Diptera: Calliphoridae)".JOURNAL OF MEDICAL ENTOMOLOGY 55.5(2018):1113-1123.
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