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Forensically Relevant Blow Flies in Lebanon Survey and Identification Using Molecular Markers (Diptera: Calliphoridae)
Shayya, Salman1,2,3,6; Debruyne, Regis4; Nel, Andre1; Azar, Dany5,6
2018-09-01
Source PublicationJOURNAL OF MEDICAL ENTOMOLOGY
ISSN0022-2585
Volume55Issue:5Pages:1113-1123
Abstract

Calliphoridae are among the first insects associated to decomposing animal remains. We have collected 1,841 specimens of three calliphorid genera: Calliphora, Lucilia, and Chrysomya, from different Lebanese localities as a first step in implementing a database of insects of forensic relevance for the country. Blow-flies are crucial for the estimation of the postmortem interval. DNA-based identification is a rapid and accurate method, often used for morphologically similar species, especially for immatures or incomplete specimens. In this study, we test the suitability of three genetic markers to identify adults and immature stages of calliphorids, viz., mitochondrial cytochrome c oxidase subunit I (COI) barcode, a region including partial sequences of mitochondrial Cyt-b-tRNA(ser)-ND1, and second internal transcribed spacer (ITS2) region of nuclear ribosomal DNA. Forty Lebanese specimens of various developmental stages (egg, larva, wandering third instar, pupa, newly emerged adult, and mature adult) were identified among the three calliphorid genera: Calliphora, Lucilia, and Chrysomya, and compared with published sequences to confirm their specific assignation. Phylogenetic analyses showed the robustness of ITS2 and COI to identify calliphorids at species level. Nevertheless, ITS2 failed to discriminate Lucilia caesar (Linnaeus) (Diptera, Calliphoridae) from Lucilia illustris (Meigen) (Diptera, Calliphoridae), and COI had a similar issue with Lucilia sericata (Meigen) (Diptera, Calliphoridae) and Lucilia cuprina (Wiedemann) (Diptera, Calliphoridae). Thus, these two markers are complementary. This work contributes new nucleotide sequences for Lebanon. It is a first step in implementing a molecular database of forensic relevant insects for the country.

KeywordForensic Entomology Cytochrome Oxidase i Second Internal Transcribed Spacer Calliphoridae Diagnostic
DOI10.1093/jme/tjy068
Language英语
WOS KeywordDna-based Identification ; Cytochrome-oxidase-i ; Mediterranean Blowflies ; Rabbit Carcasses ; 1st Instars ; Species Identification ; Lucilia-sericata ; Morphology ; Succession ; Mitochondrial
Funding ProjectCNRS (National Council of Scientific Research-Lebanon) ; W alid Joumblatt Foundation for University studies
WOS Research AreaEntomology ; Veterinary Sciences
WOS SubjectEntomology ; Veterinary Sciences
WOS IDWOS:000456130900007
Funding OrganizationCNRS (National Council of Scientific Research-Lebanon) ; W alid Joumblatt Foundation for University studies
PublisherOXFORD UNIV PRESS INC
Citation statistics
Document Type期刊论文
Identifierhttp://ir.nigpas.ac.cn/handle/332004/16070
Collection中国科学院南京地质古生物研究所
Corresponding AuthorShayya, Salman
Affiliation1.Sorbonne Univ, MNHN, UMR 7205, ISYEB,CNRS,UPMC,EPHE, 57 Rue Cuvier,CP 50, F-75005 Paris, France
2.Rafic Hariri Univ Campus, Doctoral Sch Sci & Technol, Hadath, Lebanon
3.Lebanese Atom Energy Commiss, Natl Council Sci Res Lebanon CNRS L, Beirut, Lebanon
4.Sorbonne Univ, MNHN, CNRS, Serv Systemat Mol,UMS 2700, 57 Rue Cuvier,CP 26, F-75005 Paris, France
5.Chinese Acad Sci, State Key Lab Palaeobiol & Stratig, Nanjing Inst Geol & Palaeontol, Nanjing 210008, Jiangsu, Peoples R China
6.Lebanese Univ, Fac Sci 2, Dept Nat Sci, POB 26110217, Fanar Matn, Lebanon
Recommended Citation
GB/T 7714
Shayya, Salman,Debruyne, Regis,Nel, Andre,et al. Forensically Relevant Blow Flies in Lebanon Survey and Identification Using Molecular Markers (Diptera: Calliphoridae)[J]. JOURNAL OF MEDICAL ENTOMOLOGY,2018,55(5):1113-1123.
APA Shayya, Salman,Debruyne, Regis,Nel, Andre,&Azar, Dany.(2018).Forensically Relevant Blow Flies in Lebanon Survey and Identification Using Molecular Markers (Diptera: Calliphoridae).JOURNAL OF MEDICAL ENTOMOLOGY,55(5),1113-1123.
MLA Shayya, Salman,et al."Forensically Relevant Blow Flies in Lebanon Survey and Identification Using Molecular Markers (Diptera: Calliphoridae)".JOURNAL OF MEDICAL ENTOMOLOGY 55.5(2018):1113-1123.
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