NIGPAS OpenIR
Genome-wide transcriptional response of microRNAs to the benzo(a) pyrene stress in amphioxus Branchiostoma belcheri
Zhang, Qi-Lin1; Dong, Zhi-Xiang1; Xiong, Yan1; Li, Hong-Wei1; Guo, Jun1; Wang, Feng1; Deng, Xian-Yu1; Chen, Jun-Yuan2,3; Lin, Lian-Bing1
2019-03-01
Source PublicationCHEMOSPHERE
ISSN0045-6535
Volume218Pages:205-210
AbstractAmphioxus, a cephalochordate found in sand habitats in shallow in-shore seawaters, has been widely used as a model in comparative immunology of chordates. However, the role of microRNAs (miRNAs) in amphioxus under abiotic stress, particularly xenobiotics with strong toxicity, remains largely unknown. Here, a widespread marine contaminant, benzo(a)pyrene (BaP) is used to evaluate its toxic effects on miRNA expression of amphioxus. Six small RNA libraries were sequenced from Branchiostoma belcheri. A total of 144 known and 157 novel miRNAs were identified using deep sequencing and bioinformatics approaches. A total of 58 differentially expressed miRNAs (DEMs) were screened, including 25 up- and 33 down-regulated DEMs under BaP stress. Target genes possibly regulated by DEMs were predicted, and their functional enrichment analyses were performed. Targets of DEMs are primarily involved in xenobiotic and cellular homeostasis, catabolic and transport process. They could be largely linked to nine immune- and toxin detoxification-related pathways, including metabolism of xenobiotics by cytochrome P450, drug metabolism-other enzymes, and drug metabolism-cytochrome P450, etc. Furthermore, quantitative real-time PCR (qRT-PCR) analysis for 12 key BaP-responsive DEMs validates the accuracy of deep sequencing. Experiments were then conducted to investigate their expression responses to BaP stress at different time intervals in detail to further determine their expression dynamic in responses of B. belcheri towards BaP exposure. This study, to the best of our knowledge, investigates the regulatory roles of miRNAs in the toxicological response of amphioxus for the first time, providing valuable information for the protection of lone existing cephalochordate amphioxus. (C) 2018 Elsevier Ltd. All rights reserved.
KeywordBranchiostoma belcheri miRNA Expression response Benzo(a)pyrene Deep sequencing Bioinformatics
DOI10.1016/j.chemosphere.2018.11.119
Language英语
WOS KeywordSHRIMP LITOPENAEUS-VANNAMEI ; EXPRESSION ; EXPOSURE ; GENES ; MECHANISMS ; NITRITE ; EMBRYO ; MIRNAS ; GILLS ; LIVER
Funding ProjectNatural Science Foundation of China[31760042] ; 973 project of Ministry of Science and Technology of China[2013CB835300] ; program B for Outstanding PhD candidate of Nanjing University, China[201702B075]
WOS Research AreaEnvironmental Sciences & Ecology
WOS SubjectEnvironmental Sciences
WOS IDWOS:000456640900022
Funding OrganizationNatural Science Foundation of China ; 973 project of Ministry of Science and Technology of China ; program B for Outstanding PhD candidate of Nanjing University, China
PublisherPERGAMON-ELSEVIER SCIENCE LTD
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.nigpas.ac.cn/handle/332004/16027
Collection中国科学院南京地质古生物研究所
Corresponding AuthorZhang, Qi-Lin; Lin, Lian-Bing
Affiliation1.Kunming Univ Sci & Technol, Fac Life Sci & Technol, 727 Jinming South Rd, Kunming 650500, Yunnan, Peoples R China
2.Chinese Acad Sci, Nanjing Inst Geol & Paleontol, LPS, Nanjing 210008, Jiangsu, Peoples R China
3.Nanjing Univ, Sch Life Sci, State Key Lab Pharmaceut Biotechnol, Nanjing 210023, Jiangsu, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Qi-Lin,Dong, Zhi-Xiang,Xiong, Yan,et al. Genome-wide transcriptional response of microRNAs to the benzo(a) pyrene stress in amphioxus Branchiostoma belcheri[J]. CHEMOSPHERE,2019,218:205-210.
APA Zhang, Qi-Lin.,Dong, Zhi-Xiang.,Xiong, Yan.,Li, Hong-Wei.,Guo, Jun.,...&Lin, Lian-Bing.(2019).Genome-wide transcriptional response of microRNAs to the benzo(a) pyrene stress in amphioxus Branchiostoma belcheri.CHEMOSPHERE,218,205-210.
MLA Zhang, Qi-Lin,et al."Genome-wide transcriptional response of microRNAs to the benzo(a) pyrene stress in amphioxus Branchiostoma belcheri".CHEMOSPHERE 218(2019):205-210.
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