NIGPAS OpenIR
Genome-wide gene expression analysis reveals novel insights into the response to nitrite stress in gills of Branchiostoma belcheri
Zhang, Qi-Lin1; Zhang, Guan-Ling1; Xiong, Yan1; Li, Hong-Wei1; Guo, Jun1; Wang, Feng1; Deng, Xian-Yu1; Chen, Jun-Yuan (陈均远)3,4; Wang, Yu-Jun2; Lin, Lian-Bing1
2019-03-01
Source PublicationCHEMOSPHERE
ISSN0045-6535
Volume218Pages:609-615
Abstract

Amphioxus has been widely used as a model for the comparative immunology of vertebrates. Studies have reported that gene expression changes in the amphioxus gill in response to biotic stress, such as microbial and their mimic challenge, but little is known about how gene expression is affected by abiotic stress in the marine environment, such as nitrite. A lack of information regarding gene expression response to abiotic stress hinders a comprehensive understanding of gill defense response in amphioxus. Here, RNA sequencing was used to carry out gene expression profiling analyses of Branchiostoma belched gills under nitrite stress. Six libraries were created for the control and treatment groups, including three biological replicates. In total, 2416 differently expressed genes (DEGs) were detected in response to nitrite stress, of which 1522 DEGs were up-regulated in the treatment group in comparison to the control, while the remaining 894 DEGs were down-regulated genes. Functional enrichment revealed that these DEGs are primarily involved in disease, innate immunity, xenobiotic biodegradation and metabolism, and biomolecular processes and apoptosis. We screened 11 key nitrite-responsive DEGs to detect their expression responses to nitrite stress at different time points, and validate the sequencing data using real time quantitative PCR. The results indicated that the expression of gene encoding CYP3A, POD, CASPR1, GST, MAO, DDH, and XDH/XO were induced, while those encoding MRC, GT, DNASE1L, and RIPK5 were reduced, to participate in the anti-nitrite response. This study provides a useful resource for research of molecular toxicology in amphioxus under environmental stress. (C) 2018 Elsevier Ltd. All rights reserved.

KeywordBranchiostoma Belcheri Gene Expression Profiling Gill Nitrite Molecular Toxicology Rna-seq
DOI10.1016/j.chemosphere.2018.11.156
Language英语
WOS KeywordShrimp Litopenaeus-vannamei ; Oxidative Stress ; Amphioxus ; Toxicity ; Hemocytes ; Evolution ; Apoptosis ; Exposure ; Protein ; Nitrate
Funding ProjectNatural Science Foundation of China[31760042] ; Natural Science Foundation of China[31760713] ; 973 project of Ministry of Science and Technology of China[2013CB835300] ; program B for Outstanding PhD candidate of Nanjing University[201702B075]
WOS Research AreaEnvironmental Sciences & Ecology
WOS SubjectEnvironmental Sciences
WOS IDWOS:000456640900068
Funding OrganizationNatural Science Foundation of China ; 973 project of Ministry of Science and Technology of China ; program B for Outstanding PhD candidate of Nanjing University
PublisherPERGAMON-ELSEVIER SCIENCE LTD
Citation statistics
Document Type期刊论文
Identifierhttp://ir.nigpas.ac.cn/handle/332004/16011
Collection中国科学院南京地质古生物研究所
其他
Corresponding AuthorZhang, Qi-Lin; Wang, Yu-Jun; Lin, Lian-Bing
Affiliation1.Kunming Univ Sci & Technol, Fac Life Sci & Technol, Kunming 650500, Yunnan, Peoples R China
2.Qinzhou Univ, Ocean Coll, Guangxi Key Lab Beibu Gulf Marine Biodivers Conse, Qinzhou 535011, Peoples R China
3.Chinese Acad Sci, Nanjing Inst Geol & Paleontol, LPS, Nanjing 210008, Jiangsu, Peoples R China
4.Nanjing Univ, Sch Life Sci, State Key Lab Pharmaceut Biotechnol, Nanjing 210023, Jiangsu, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Qi-Lin,Zhang, Guan-Ling,Xiong, Yan,et al. Genome-wide gene expression analysis reveals novel insights into the response to nitrite stress in gills of Branchiostoma belcheri[J]. CHEMOSPHERE,2019,218:609-615.
APA Zhang, Qi-Lin.,Zhang, Guan-Ling.,Xiong, Yan.,Li, Hong-Wei.,Guo, Jun.,...&Lin, Lian-Bing.(2019).Genome-wide gene expression analysis reveals novel insights into the response to nitrite stress in gills of Branchiostoma belcheri.CHEMOSPHERE,218,609-615.
MLA Zhang, Qi-Lin,et al."Genome-wide gene expression analysis reveals novel insights into the response to nitrite stress in gills of Branchiostoma belcheri".CHEMOSPHERE 218(2019):609-615.
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