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Comparative transcriptomic analysis provides insights into the response to the benzo(a)pyrene stress in aquatic firefly (Luciola leii)
Zhang, Qi-Lin1; Guo, Jun1; Deng, Xian-Yu1; Wang, Feng1; Chen, Jun-Yuan (陈均远)2; Lin, Lian-Bing1
2019-04-15
Source PublicationSCIENCE OF THE TOTAL ENVIRONMENT
ISSN0048-9697
Volume661Pages:226-234
Abstract

Many studies have reported that behavior and bioluminescence of fireflies could be affected by changes in environment conditions. However, little is known about how the deterioration of the aquatic environment affects aquatic fireflies, particularly with respect to molecular responses following exposure to water pollutants, such as benzo(a)pyrene (BaP), which is a key indicator in environmental risk assessment because of the hazards it poses. Here, whole transcriptome sequencing and gene expression analysis were performed on freshwater fire-flies (Luciola leii) exposed to BaP (concentration of 0.01mg/L). Four transcriptomic librarieswere constructed for the control and treatment groups, including two biological replicates. From the mixed pools (each pool contains 60 individuals from three time points), a total of 54,282 unigenes were assembled. Furthermore, 329,337 of Single-nucleotide Polymorphisms (SNPs) and 1324 of Simple Sequence Repeats (SSRs) were predicted using bioinformatics, which is useful for the future development of molecular markers. Subsequently, 2414 differently expressed genes (DEGs) were identified in response to BaP stress in comparison to the control, including 1350 up-regulated and 1064 down-regulated DEGs. Functional enrichment showed that these DEGs are primarily related to innate immunity; xenobiotic biodegradation and response, biomacromolecule metabolism, biosynthesis, and absorption. Eight key BaP-responsive DEGs were screened to survey the dynamic changes of expression in response to BaP stress at different time points, and to validate the RNA sequencing data using quantitative real-time PCR. The results indicate that the expression of genes encoding UGT, CYP3A, CYP9, CYP6AS5 and ADHP were induced, while those encoding UGT2B10L, PTGDS, and ALDH were reduced, to participate in response to the BaP exposure and potentially help counteract the adverse effects of BaP. This investigation provides insight into the toxicological response of fireflies to the occurrence of water deterioration. (C) 2019 Elsevier B.V. All rights reserved.

KeywordLuciola Leii Larvae Transcriptomics Gene Expression Benzo(a)Pyrene Molecular Toxicology Rna Sequencing
DOI10.1016/j.scitotenv.2019.01.156
Language英语
WOS KeywordGene-expression Analysis ; Shrimp Litopenaeus-vannamei ; Rna ; Hypoxia ; Nitrite ; Annotation ; Generation ; Drosophila ; Patterns ; Exposure
Funding ProjectNatural Science Foundation of China[31760042] ; Natural Science Foundation of China[31760713]
WOS Research AreaEnvironmental Sciences & Ecology
WOS SubjectEnvironmental Sciences
WOS IDWOS:000458408200023
Funding OrganizationNatural Science Foundation of China
PublisherELSEVIER SCIENCE BV
Citation statistics
Document Type期刊论文
Identifierhttp://ir.nigpas.ac.cn/handle/332004/15996
Collection中国科学院南京地质古生物研究所
其他
Corresponding AuthorZhang, Qi-Lin; Lin, Lian-Bing
Affiliation1.Kunming Univ Sci & Technol, Fac Life Sci & Technol, Kunming 650500, Yunnan, Peoples R China
2.Chinese Acad Sci, Nanjing Inst Geol & Paleontol, LPS, Nanjing 210008, Jiangsu, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Qi-Lin,Guo, Jun,Deng, Xian-Yu,et al. Comparative transcriptomic analysis provides insights into the response to the benzo(a)pyrene stress in aquatic firefly (Luciola leii)[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2019,661:226-234.
APA Zhang, Qi-Lin,Guo, Jun,Deng, Xian-Yu,Wang, Feng,Chen, Jun-Yuan ,&Lin, Lian-Bing.(2019).Comparative transcriptomic analysis provides insights into the response to the benzo(a)pyrene stress in aquatic firefly (Luciola leii).SCIENCE OF THE TOTAL ENVIRONMENT,661,226-234.
MLA Zhang, Qi-Lin,et al."Comparative transcriptomic analysis provides insights into the response to the benzo(a)pyrene stress in aquatic firefly (Luciola leii)".SCIENCE OF THE TOTAL ENVIRONMENT 661(2019):226-234.
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