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Identification of circular RNAs and their altered expression under poly(I:C) challenge in key antiviral immune pathways in amphioxus
Zhang, Qi-Lin1; Ji, Xin-Yu1; Li, Hong-Wei1; Guo, Jun1; Wang, Feng1; Deng, Xian-Yu1; Chen, Jun-Yuan (陈均远)2,3; Lin, Lian-Bing1
2019-03-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume86Pages:1053-1057
Abstract

Amphioxus is a key model for studying comparative immunity of vertebrates. Circular RNA (circRNA), as RNAs with a circular structure, has received little attention until recently, where several studies have reported that circRNA expression changes are involved in the immune response in animals. However, circRNA and its immune role in amphioxus have not been previously studied. Here, circRNAs in Chinese amphioxus (Branchiostoma belcheri) were sequenced, and 1859 circRNAs were identified using two algorithms (find_circ and CIRI). The analysis of miRNA target sites on circRNAs showed that 332 circRNAs may function as miRNA sponges. Furthermore, we identified circRNAs that were conserved between B. belcheri and vertebrates, tracing the origin of these circRNAs within chordates. Additionally, in combination with several key antiviral immune (poly(I:C), pIC) pathways identified in our previous B. belcheri studies, nine circRNAs potentially involved in these pathways were identified using bioinformatic predictions. Among these nine circRNAs, eight were selected to examine their expression response in B. belcheri challenged by pIC in comparison to control using real-time quantitative PCR. The results showed that four circRNAs were induced as part of the antiviral response against pIC, while expression of two circRNAs was decreased, and the expression levels of the remaining two were not significantly altered after pIC challenge. This work is the first to identify circRNAs and reveal their antiviral role in amphioxus. Therefore, it opens a new window to explore the comparative immunology of circRNAs in chordates and the regulatory roles of circRNAs in antiviral immunity in amphioxus.

KeywordBranchiostoma Belcheri Circular Rna-seq Poly (i:c) Antiviral Immune Pathways Qrt-pcr
DOI10.1016/j.fsi.2018.12.061
Language英语
WOS KeywordMicrorna ; Genome ; Efficient ; Evolution ; Pcr
Funding Project973 project of Ministry of Science and Technology of China[2013CB835300] ; Natural Science Foundation of China[31760042]
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000459528400116
Funding Organization973 project of Ministry of Science and Technology of China ; Natural Science Foundation of China
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Citation statistics
Document Type期刊论文
Identifierhttp://ir.nigpas.ac.cn/handle/332004/15967
Collection中国科学院南京地质古生物研究所
其他
Corresponding AuthorLin, Lian-Bing
Affiliation1.Kunming Univ Sci & Technol, Fac Life Sci & Technol, Kunming 650500, Yunnan, Peoples R China
2.Chinese Acad Sci, Nanjing Inst Geol & Paleontol, LPS, Nanjing 210008, Peoples R China
3.Nanjing Univ, Sch Life Sci, State Key Lab Pharmaceut Biotechnol, Nanjing 210023, Jiangsu, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Qi-Lin,Ji, Xin-Yu,Li, Hong-Wei,et al. Identification of circular RNAs and their altered expression under poly(I:C) challenge in key antiviral immune pathways in amphioxus[J]. FISH & SHELLFISH IMMUNOLOGY,2019,86:1053-1057.
APA Zhang, Qi-Lin.,Ji, Xin-Yu.,Li, Hong-Wei.,Guo, Jun.,Wang, Feng.,...&Lin, Lian-Bing.(2019).Identification of circular RNAs and their altered expression under poly(I:C) challenge in key antiviral immune pathways in amphioxus.FISH & SHELLFISH IMMUNOLOGY,86,1053-1057.
MLA Zhang, Qi-Lin,et al."Identification of circular RNAs and their altered expression under poly(I:C) challenge in key antiviral immune pathways in amphioxus".FISH & SHELLFISH IMMUNOLOGY 86(2019):1053-1057.
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