NIGPAS OpenIR
MicroRNA profile of immune response in gills of zebrafish (Danio rerio) upon Staphylococcus aureus infection
Zhang, Qi-Lin1; Dong, Zhi-Xiang1; Luo, Zhi-Wen1; Jiao, Yun-Jian1; Guo, Jun1; Deng, Xian-Yu1; Wang, Feng1; Chen, Jun-Yuan (陈均远)2,3; Lin, Lian-Bing1
2019-04-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume87Pages:307-314
Abstract

The gills of fish are large mucosal surfaces that are very important portals for pathogen entry. Investigations have shown that microRNAs (miRNAs) are key regulators of immune response to bacterial infections in the gills of fish; however, how miRNA expression changes in response to infection by Gram-positive bacteria remains largely unknown. To further investigate the immunological role of miRNAs in fish gills under pathogen stress induced by Gram-positive bacterial infection, this study investigated Staphylococcus aureus (SA)-induced changes in the miRNAs levels in gills of adult zebrafish (Danio rerio). miRNA microarrays were used to analyze expression profiles of known miRNA in the gills of zebrafish in response to SA infection and compared these to uninfected control fish. A total of 30 differentially expressed miRNAs (DEMs) were identified. Target genes likely regulated by DEMs were predicted, and functional enrichment analyses were performed. The results indicated that DEM targets were primarily involved in innate immune processes, apoptosis, defense responses, and antibacterial responses. Pathways involving bacterial infection, innate immunity, metabolic process, disease, and apoptosis were mediated by DEMs. Furthermore, real-time quantitative PCR experiments for nine key SA-responsive DEMs that regulated the "SA infection" pathway validated the accuracy of microarray results. Dynamic variations in gene expression were surveyed in detail for these key SA-responsive DEMs for PBS control and at 6, 12, 24, and 48 h after SA challenge in detail. This study provides novel insight into the mechanisms underlying the miRNA regulation during the SA-induced immune response in zebrafish gills, and provides basic knowledge on the innate immune response against Gram-positive bacterial infection in bony fish.

KeywordZebrafish Micrornas Gill Staphylococcus Aureus Microarray Qrt-pcr
DOI10.1016/j.fsi.2019.01.026
Language英语
WOS KeywordCynoglossus-semilaevis ; Vibrio-anguillarum ; Innate Immunity ; Up-regulation ; Expression ; Exposure ; Fish ; Skin ; Identification ; Activation
Funding ProjectNatural Science Foundation of China[31760042] ; 973 project of Ministry of Science and Technology of China[2013CB835300]
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000464086600034
Funding OrganizationNatural Science Foundation of China ; 973 project of Ministry of Science and Technology of China
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Citation statistics
Document Type期刊论文
Identifierhttp://ir.nigpas.ac.cn/handle/332004/15787
Collection中国科学院南京地质古生物研究所
其他
Corresponding AuthorZhang, Qi-Lin; Lin, Lian-Bing
Affiliation1.Kunming Univ Sci & Technol, Fac Life Sci & Technol, Kunming 650500, Yunnan, Peoples R China
2.Chinese Acad Sci, Nanjing Inst Geol & Paleontol, LPS, Nanjing 210008, Jiangsu, Peoples R China
3.Nanjing Univ, Sch Life Sci, State Key Lab Pharmaceut Biotechnol, Nanjing 210023, Jiangsu, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Qi-Lin,Dong, Zhi-Xiang,Luo, Zhi-Wen,et al. MicroRNA profile of immune response in gills of zebrafish (Danio rerio) upon Staphylococcus aureus infection[J]. FISH & SHELLFISH IMMUNOLOGY,2019,87:307-314.
APA Zhang, Qi-Lin.,Dong, Zhi-Xiang.,Luo, Zhi-Wen.,Jiao, Yun-Jian.,Guo, Jun.,...&Lin, Lian-Bing.(2019).MicroRNA profile of immune response in gills of zebrafish (Danio rerio) upon Staphylococcus aureus infection.FISH & SHELLFISH IMMUNOLOGY,87,307-314.
MLA Zhang, Qi-Lin,et al."MicroRNA profile of immune response in gills of zebrafish (Danio rerio) upon Staphylococcus aureus infection".FISH & SHELLFISH IMMUNOLOGY 87(2019):307-314.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhang, Qi-Lin]'s Articles
[Dong, Zhi-Xiang]'s Articles
[Luo, Zhi-Wen]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhang, Qi-Lin]'s Articles
[Dong, Zhi-Xiang]'s Articles
[Luo, Zhi-Wen]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhang, Qi-Lin]'s Articles
[Dong, Zhi-Xiang]'s Articles
[Luo, Zhi-Wen]'s Articles
Terms of Use
No data!
Social Bookmark/Share
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit Add to Technorati
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.