NIGPAS OpenIR
MicroRNA profile of immune response in gills of zebrafish (Danio rerio) upon Staphylococcus aureus infection
Zhang, Qi-Lin1; Dong, Zhi-Xiang1; Luo, Zhi-Wen1; Jiao, Yun-Jian1; Guo, Jun1; Deng, Xian-Yu1; Wang, Feng1; Chen, Jun-Yuan (陈均远)2,3; Lin, Lian-Bing1
2019-04-01
发表期刊FISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
卷号87页码:307-314
摘要

The gills of fish are large mucosal surfaces that are very important portals for pathogen entry. Investigations have shown that microRNAs (miRNAs) are key regulators of immune response to bacterial infections in the gills of fish; however, how miRNA expression changes in response to infection by Gram-positive bacteria remains largely unknown. To further investigate the immunological role of miRNAs in fish gills under pathogen stress induced by Gram-positive bacterial infection, this study investigated Staphylococcus aureus (SA)-induced changes in the miRNAs levels in gills of adult zebrafish (Danio rerio). miRNA microarrays were used to analyze expression profiles of known miRNA in the gills of zebrafish in response to SA infection and compared these to uninfected control fish. A total of 30 differentially expressed miRNAs (DEMs) were identified. Target genes likely regulated by DEMs were predicted, and functional enrichment analyses were performed. The results indicated that DEM targets were primarily involved in innate immune processes, apoptosis, defense responses, and antibacterial responses. Pathways involving bacterial infection, innate immunity, metabolic process, disease, and apoptosis were mediated by DEMs. Furthermore, real-time quantitative PCR experiments for nine key SA-responsive DEMs that regulated the "SA infection" pathway validated the accuracy of microarray results. Dynamic variations in gene expression were surveyed in detail for these key SA-responsive DEMs for PBS control and at 6, 12, 24, and 48 h after SA challenge in detail. This study provides novel insight into the mechanisms underlying the miRNA regulation during the SA-induced immune response in zebrafish gills, and provides basic knowledge on the innate immune response against Gram-positive bacterial infection in bony fish.

关键词Zebrafish Micrornas Gill Staphylococcus Aureus Microarray Qrt-pcr
DOI10.1016/j.fsi.2019.01.026
语种英语
关键词[WOS]Cynoglossus-semilaevis ; Vibrio-anguillarum ; Innate Immunity ; Up-regulation ; Expression ; Exposure ; Fish ; Skin ; Identification ; Activation
资助项目Natural Science Foundation of China[31760042] ; 973 project of Ministry of Science and Technology of China[2013CB835300]
WOS研究方向Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS类目Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号WOS:000464086600034
项目资助者Natural Science Foundation of China ; 973 project of Ministry of Science and Technology of China
出版者ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
文献类型期刊论文
条目标识符http://ir.nigpas.ac.cn/handle/332004/15787
专题中国科学院南京地质古生物研究所
其他
通讯作者Zhang, Qi-Lin; Lin, Lian-Bing
作者单位1.Kunming Univ Sci & Technol, Fac Life Sci & Technol, Kunming 650500, Yunnan, Peoples R China
2.Chinese Acad Sci, Nanjing Inst Geol & Paleontol, LPS, Nanjing 210008, Jiangsu, Peoples R China
3.Nanjing Univ, Sch Life Sci, State Key Lab Pharmaceut Biotechnol, Nanjing 210023, Jiangsu, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Qi-Lin,Dong, Zhi-Xiang,Luo, Zhi-Wen,et al. MicroRNA profile of immune response in gills of zebrafish (Danio rerio) upon Staphylococcus aureus infection[J]. FISH & SHELLFISH IMMUNOLOGY,2019,87:307-314.
APA Zhang, Qi-Lin.,Dong, Zhi-Xiang.,Luo, Zhi-Wen.,Jiao, Yun-Jian.,Guo, Jun.,...&Lin, Lian-Bing.(2019).MicroRNA profile of immune response in gills of zebrafish (Danio rerio) upon Staphylococcus aureus infection.FISH & SHELLFISH IMMUNOLOGY,87,307-314.
MLA Zhang, Qi-Lin,et al."MicroRNA profile of immune response in gills of zebrafish (Danio rerio) upon Staphylococcus aureus infection".FISH & SHELLFISH IMMUNOLOGY 87(2019):307-314.
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