NIGPAS OpenIR  > 中国科学院南京地质古生物研究所
microRNA expression changes after lipopolysaccharide treatment in gills of amphioxus Branchiostoma belcheri
Liao, X; Yang, L; Zhang, QL; Chen, JY (陈均远)
2017
Source PublicationDevelopmental and Comparative Immunology
ISSN0145-305X
Volume70Pages:39-44
AbstractRecently, amphioxus has served as a model for studying the origin and evolution of vertebrate immunity. However, little is known about how microRNAs (miRNAs) are involved in the immune defense in amphioxus. In this article, we identified the amphioxus miRNAs in the acute-phase response to lipopolysaccharide (LPS). We determined the time point for the peak of immune response in amphioxus after LPS challenge by evaluating the expression of Branchiostoma belcheri toll-like receptor 1, NF-icb (c-Rel), and big defensin which react with pathogen-associated molecular pattems(PAMPs). Then we chose 12 h as the point to perform miRNA microarray analysis to select the differentially expressed miRNAs. Furthermore, we used quantitative real-time PCR to detect the expression patterns of selected amphioxus miRNAs under effective LPS challenge during the time course. The microarray data revealed that the miRNA expression file was significantly changed after LPS stimulation. The changes of the 10 most upregulated and 7 most downregualted miRNAs in gills of the amphioxus following challenge with LPS revealed a temporal induction kinetic. Our current study will provide valuable information to take an insight into molecular mechanism of innate immune and the evolution of the miRNA family. (C) 2017 Elsevier Ltd. All rights reserved.
KeywordmicroRNA Innate immune Branchiostoma belcheri Lipopolysaccharide
DOI10.1016/j.dci.2017.01.007
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Immunology ; Zoology
WOS SubjectFisheries ; Immunology ; Zoology
WOS IDWOS:000394635500005
PublisherELSEVIER SCI LTD
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.nigpas.ac.cn/handle/332004/13653
Collection中国科学院南京地质古生物研究所
其他
Corresponding AuthorChen, JY (陈均远)
Recommended Citation
GB/T 7714
Liao, X,Yang, L,Zhang, QL,et al. microRNA expression changes after lipopolysaccharide treatment in gills of amphioxus Branchiostoma belcheri[J]. Developmental and Comparative Immunology,2017,70:39-44.
APA Liao, X,Yang, L,Zhang, QL,&Chen, JY .(2017).microRNA expression changes after lipopolysaccharide treatment in gills of amphioxus Branchiostoma belcheri.Developmental and Comparative Immunology,70,39-44.
MLA Liao, X,et al."microRNA expression changes after lipopolysaccharide treatment in gills of amphioxus Branchiostoma belcheri".Developmental and Comparative Immunology 70(2017):39-44.
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