microRNA expression changes after lipopolysaccharide treatment in gills of amphioxus Branchiostoma belcheri

doi:10.1016/j.dci.2017.01.007

	microRNA expression changes after lipopolysaccharide treatment in gills of amphioxus Branchiostoma belcheri
	Liao, X; Yang, L; Zhang, QL; Chen, JY (陈均远)
	2017
发表期刊	Developmental and Comparative Immunology
ISSN	0145-305X
卷号	70 页码:39-44
摘要	Recently, amphioxus has served as a model for studying the origin and evolution of vertebrate immunity. However, little is known about how microRNAs (miRNAs) are involved in the immune defense in amphioxus. In this article, we identified the amphioxus miRNAs in the acute-phase response to lipopolysaccharide (LPS). We determined the time point for the peak of immune response in amphioxus after LPS challenge by evaluating the expression of Branchiostoma belcheri toll-like receptor 1, NF-icb (c-Rel), and big defensin which react with pathogen-associated molecular pattems(PAMPs). Then we chose 12 h as the point to perform miRNA microarray analysis to select the differentially expressed miRNAs. Furthermore, we used quantitative real-time PCR to detect the expression patterns of selected amphioxus miRNAs under effective LPS challenge during the time course. The microarray data revealed that the miRNA expression file was significantly changed after LPS stimulation. The changes of the 10 most upregulated and 7 most downregualted miRNAs in gills of the amphioxus following challenge with LPS revealed a temporal induction kinetic. Our current study will provide valuable information to take an insight into molecular mechanism of innate immune and the evolution of the miRNA family. (C) 2017 Elsevier Ltd. All rights reserved.
关键词	microRNA Innate immune Branchiostoma belcheri Lipopolysaccharide
DOI	10.1016/j.dci.2017.01.007
收录类别	SCI
语种	英语
WOS研究方向	Fisheries ; Immunology ; Zoology
WOS类目	Fisheries ; Immunology ; Zoology
WOS记录号	WOS:000394635500005
出版者	ELSEVIER SCI LTD
文献类型	期刊论文
条目标识符	http://ir.nigpas.ac.cn/handle/332004/13653
专题	中国科学院南京地质古生物研究所其他
通讯作者	Chen, JY (陈均远)
推荐引用方式 GB/T 7714	Liao, X,Yang, L,Zhang, QL,et al. microRNA expression changes after lipopolysaccharide treatment in gills of amphioxus Branchiostoma belcheri[J]. Developmental and Comparative Immunology,2017,70:39-44.
APA	Liao, X,Yang, L,Zhang, QL,&Chen, JY .(2017).microRNA expression changes after lipopolysaccharide treatment in gills of amphioxus Branchiostoma belcheri.Developmental and Comparative Immunology,70,39-44.
MLA	Liao, X,et al."microRNA expression changes after lipopolysaccharide treatment in gills of amphioxus Branchiostoma belcheri".Developmental and Comparative Immunology 70(2017):39-44.